| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.25[luc2CP/minP] |
| 质粒类型: | 荧光素酶表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 4462 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 具有最小启动子的载体，用于使用高度不稳定的荧光素酶测量转录反应元件的活性。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pGL4.25[luc2CP/minP]载体质粒基本信息
pGL4.25[luc2CP/minP]质粒图谱载体图谱和pGL4.25[luc2CP/minP]载体序列质粒序列多克隆位点信息
![pGL4.25[luc2CP-minP]载体图谱](/uploads/载体图谱/pGL4.25[luc2CP-minP]载体图谱.png)
pGL4.25[luc2CP/minP]质粒载体简介
pGL4.25[luc2CP/minP]质粒序列载体序列
LOCUS pGL4.25[luc2CP minP] 4462 bp ds-DNA circular SYN 29-JUN-2013
DEFINITION Vector with a minimal promoter for measuring the activity of
transcriptional response elements using highly destabilized
luciferase.
ACCESSION DQ904457
VERSION .
KEYWORDS pGL4.25[luc2CP minP]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4462)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Saturday, June 13, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4462
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
promoter 77..108
/note="minP"
/note="minimal TATA-box promoter with low basal activity"
CDS 141..1790
/codon_start=1
/product="firefly luciferase"
/note="luciferase"
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
CDS 1797..1844
/codon_start=1
/product="non-ORF yeast peptide conferring
ubiquitin-dependent degradation"
/note="hCL1"
/note="human codon-optimized"
/translation="ACKNWFSSLSHFVIHL"
CDS 1848..1967
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/note="hPEST"
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
polyA_signal 2016..2137
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2556..3144)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3344..4204)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 4309..4357
/note="synthetic polyadenylation signal"
misc_feature 4371..4462
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
121 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
181 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
241 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg
301 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata
361 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg
421 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
481 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
541 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata
601 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
661 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
721 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca
781 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg
841 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
901 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
961 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
1021 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca
1081 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac
1141 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg
1201 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
1261 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg
1321 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca
1381 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
1441 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
1501 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg
1561 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga
1621 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
1681 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca
1741 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg aattctgctt
1801 gcaagaactg gttcagtagc ttaagccact ttgtgatcca ccttaacagc cacggcttcc
1861 ctcccgaggt ggaggagcag gccgccggca ccctgcccat gagctgcgcc caggagagcg
1921 gcatggatag acaccctgct gcttgcgcca gcgccaggat caacgtctaa ctgcagtcta
1981 gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
2041 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
2101 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
2161 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
2221 atgtggtaaa atcgataagg atccgtcgac cgatgccctt gagagccttc aacccagtca
2281 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
2341 tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg
2401 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
2461 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
2521 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
2581 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
2641 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
2701 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
2761 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
2821 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
2881 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
2941 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
3001 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
3061 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
3121 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
3181 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
3241 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
3301 acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag tgcttgatca
3361 gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc tgactccccg
3421 tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca gcaatgatgc
3481 cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca gcagggaggg
3541 ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg agctgctgtc
3601 gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg gccattgcta
3661 ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct ggttcccagc
3721 ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc tccttagggc
3781 ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta atggcagcac
3841 tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc ggcgagtact
3901 caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta
3961 tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc gggaatcgtt
4021 cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg atatagccca
4081 ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa
4141 aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc
4201 tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag tacgtctctc
4261 aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa taaaatatct
4321 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct
4381 ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt
4441 gcaggtgcca gaacatttct ct
//
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